- Title
- Enhancement in brain uptake of vitamin D3 nanoemulsion for treatment of cerebral ischemia: formulation, gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat models
- Creator
- Kumar, Mukesh; Nishad, Dhruv Kumar; Sharma, Nitin; Kumar, Anoop; Bhatnagar, Aseem; Karwasra, Ritu; Khanna, Kushagra; S, Keerthana; Sharma, Deeksha; Dua, Kamal; Mudaliyar, Vijaybabu
- Relation
- Journal of Microencapsulation Vol. 37, Issue 7, p. 492-501
- Publisher Link
- http://dx.doi.org/10.1080/02652048.2020.1801870
- Publisher
- Taylor & Francis
- Resource Type
- journal article
- Date
- 2020
- Description
- Aim: For the treatment of cerebral ischaemia, vitamin-D3 loaded nanoemulsions were developed. Method: Tween 20 and polyethylene glycol were chosen as surfactant/co-surfactant, while oleic acid as the oil phase. The formulation was characterised for various in-vitro parameters. Targeting efficiency was investigated through radiometry, gamma scintigraphy and efficacy was studied in transient middle cerebral artery occlusion (MCAo) rat model. Result: Vitamin D3-nanoemulsion showed a mean size range of 49.29 ± 10.28 nm with polydispersity index 0.17 ± 0.04 and zeta potential 13.77 mV. The formulation was found stable during thermodynamic stability study and permeated within 180 min through sheep nasal mucosa (permeation coefficient 7.873 ± 0.884 cm/h). Gamma scintigraphy and radiometry assay confirmed better percentage deposition (2.53 ± 0.17%) of 99mTc-vitamin D3-nanoemulsion through nasal route compared to IV administered 99mTc-vitamin D3 solution (0.79 ± 0.03%). Magnetic Resonance Imaging (MRI) of the ischaemic model confirmed better efficacy of vitamin D3-nanoemulsion. Conclusion: This work demonstrated better permeation, deposition, and efficacy of vitamin D3-nanoemulsion through the intranasal route.
- Subject
- gamma scintigraphy; ischaemia; MRI; nanoemulsion; vitamin D3
- Identifier
- http://hdl.handle.net/1959.13/1464931
- Identifier
- uon:47155
- Identifier
- ISSN:0265-2048
- Language
- eng
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